|
KMID : 0881720150300030272
|
|
Journal of Food Hygiene and Safety
2015 Volume.30 No. 3 p.272 ~ p.280
|
|
|
Development of an Official Analytical Method for Determination of Phorate and its Metabolites in Livestock Using LC-MS/MS
|
|
Ko Ah-Young
Kim Hee-Jeong
Jang Jin
Lee Eun-Hyang
Ju Yun-ji
Noh Mi-Jung
Kim Seong-Cheol
Chang Moon-Ik
Rhee Gyu-Sik
Park Sung-Won
|
|
|
Abstract
|
|
|
|
A simultaneous official method was developed for the determination of phorate and its metabolites (phorate sulfoxide, phorate sulfone, phorate oxon, phorate oxon sulfoxide, phorate oxon sulfone) in livestock samples. The analytes were quantified and confirmed via liquid chromatograph-tandem mass spectrometer (LC-MS/MS) in positive ion mode using multiple reaction monitoring (MRM). Phorate and its metabolites were extracted from beef and milk samples with acidified acetonitrile (containing 1% acetic acid) and partitioned with anhydrous magnesium sulfate. Then, the extract was purified through primary secondary amine (PSA) and C18 dispersive sorbent. Matrix matched calibration curves were linear over the calibration ranges (0.005-0.5 mg/L) for all the analytes into blank extract with r 2 > 0.996. For validation purposes, recovery studies were carried out at three different concentration levels (beef 0.004, 0.04 and 0.2 mg/kg; milk 0.008, 0.04 and 0.2 mg/kg, n = 5). The recoveries were within 79.2-113.9% with relative standard deviations (RSDs) less than 19.2% for all analytes. All values were consistent with the criteria ranges requested in the Codex guidelines. The limit of quantification was quite lower than the maximum residue limit (MRL) set by the Ministry of Food and Drug Safety (0.05 mg/kg). The proposed analytical method was accurate, effective and sensitive for phorate and its metabolites determination and it will be used to as an official analytical method in Korea.
|
|
|
KEYWORD
|
|
|
Phorate, Metabolite, Maximum residue limit, Official analytical method
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|